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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: A multifunctional ‘golden cicada’ nanoplatform breaks the thermoresistance barrier to launch cascade augmented synergistic effects of photothermal/gene therapy

Fig. 3

Cellular uptake analysis and endosomal escape of MGCN. (A) Cellular uptake analysis of PEI 1.8 K/pDNA, PEI 25 K/pDNA, PEI-FPBA/pDNA, PEI-FPBA/pDNA + SA blocked, HPI/PEI-FPBA/pDNA, and HPI/PEI-FPBA/pDNA + CD44 blocked using flow cytometry in B16-F10 cells. (B) Quantitative analysis of cellular uptake efficiency and (C) mean fluorescence intensity (MFI) in B16-F10 cells. D. The endo/lysosomal escape process of MGCN was shown in B16-F10 cells. Pictures were obtained by CLMS at 0.5 h, 2 h, 4 and 8 h, respectively. HSP70-shRNA plasmids were labeled with YOYO-1 (green), nuclei were counterstained with DAPI (blue), and endo/lysosomes were counterstained with LysoTracker Red (red). The scale bar represented 20 μm. E. Cellular uptake and distribution of ICG in B16-F10 cells. The scale bar represented 20 μm. **P < 0.01, ***P < 0.001

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Journal of Nanobiotechnology

ISSN: 1477-3155

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