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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: A multifunctional ‘golden cicada’ nanoplatform breaks the thermoresistance barrier to launch cascade augmented synergistic effects of photothermal/gene therapy

Fig. 5

Photothermal effect of MGCN in vitro. After nanocomplex administration, B16-F10 cells were further maintained for another 24 h and then received photothermal therapy. (A) Photothermal effect in B16-F10 cells after different treatments measured by MTT. (B) Fluorescence images of live/dead staining of B16-F10 cells taken by fluorescence microscopy after treated with different nanocomplexes. Living cells were counterstained with FITC (green), nuclei were counterstained with PI (red). The scale bar represented 500 μm. (C) Apoptosis analysis of B16-F10 cells determined with Annexin V-FITC/PI kit by flow cytometry. (D) Quantitative analysis of the apoptosis rate in B16-F10 cells. ***P < 0.001

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