Fig. 8

UTX/miR-467b-3p activates the PTEN/PI3K/mTOR pathway to mediate macrophage polarization. A Western blotting analysis of the expression levels of PTEN/PI3K/ mTOR signaling pathway-related proteins after miR-467b-3p mimic and inhibitor transfection of LPS-stimulated macrophages. B Statistical analysis of the expression levels of the PTEN/PI3K/mTOR signaling pathway-related proteins in A. C Western blotting analysis of the expression levels of iNOS and Arginase-1 of LPS-stimulated macrophages in Vehicle, UTX^−/−-Exos, UTX^−/−-Exos mimic, UTX^−/−-Exos inhibitor, and UTX^−/−-Exos with rapamycin groups. D, E Statistical analysis of iNOS and Arginase-1 expression levels in C. ^#P < 0.01 vs. Vehicle group, *P < 0.01 vs. UTX^−/−-Exos group. F Immunofluorescence analysis of LPS-stimulated macrophages (green) phenotypes (red) in Vehicle, UTX^−/−-Exos, UTX^−/−-Exos mimic, UTX^−/−-Exos inhibitor, and UTX^−/−-Exos with rapamycin groups. Scale bar, 20 μm. G, H Statistical analysis of the mean fluorescence intensity of CD86 and CD206 in F. ^#P < 0.01 vs. Vehicle group, ^&P < 0.05 vs. UTX^−/−-Exos group, *P < 0.01 vs. UTX^−/−-Exos group