Fig. 2
Cell uptake and anti-tumor effect in vitro. (a) Confocal laser-scanning microscopy observation of Cy5-labeled PtPDs uptake by MB49 cells. scale bar: 30 μm. (b) MB49 cell viability after cultivation for 24 h with different formulations with or without 808 nm laser exposure (0.5 W, 10 min). (c) Calcein-AM/PI staining, and (d) quantitative analysis of living cell rate in MB49 cells after treating with different formulations. Scale bar: 50 μm. (e) Percentage of CRT-positive cells. (f) Mature BMDC percentage (CD11c+CD40+) after co-incubation with treated MB49 cells for 24 h. All results represent the mean ± SD (n = 3). (g) The levels of TNF-⍺ in suspension of matured BMDCs. *p < 0.05, **p < 0.01. All results represent the mean ± SD (n = 3)