Fig. 5

Anti-tumor therapeutic effects of PtPDs in a bilateral MB49 tumor model. Tumor growth curves of (a) primary and (b) distant tumors (n = 5). (c) Survival curves after treatment. Rechallenged tumor growth curves in mice bearing 4T1 tumors following the indicated treatments (n = 5). (d) Representative flow cytometric plots of intratumoral infiltration of cytotoxic CD8⁺ T cells (CD45⁺CD3⁺CD8⁺, gated on CD45⁺ T cells) and CD4⁺ T cells (CD45⁺CD3⁺CD4⁺, gated on CD45⁺ T cells), and (e) ratio of CD8⁺/CD4⁺ T cell in distant tumor tissues isolated at fifth day post-treatment (n = 5). d) Secretion levels of TNF-α in serum after various treatments. *p < 0.05, **p < 0.01