Fig. 2
From: Nanoparticles systemically biodistribute to regenerating skeletal muscle in DMD
Dystrophic muscles differ in their degenerative/regenerative states which correlates to MSNP biodistribution. A. Representative microscopy images of CD31 immunofluorescence (IF) staining of tibialis anterior (TA) and gastrocnemius (Gastroc) in 3-months male C57BL/6 mice, mdx and mdx-DBA/2 mice. Color code: green, Cd31; blue, nuclear. Scale bar represents 50 μm. Histogram analysis of Cd31 + blood vessel density and size shows quantification from more than 8 randomly selected regions. Data represent mean ± SEM. *P < 0.05, 1-way ANOVA followed by Tukey’s test. B. αSMA labels blood vessels and myofibers independent of Cd31 in mdx muscle. Bars represents 100 μm. C. αSMA co-localizes with regenerating mdx myofibers. Scale Bar represents 50 μm