Fig. 2
Studies of uptake mechanism and transepithelial transport. (a) Cell uptake of free DOX, DS NPs and SC@DS NPs at 2 and 4Â h incubation, respectively. (b) The result of flow cytometry statistical analysis after the Caco-2 cells being incubated with DS NPs and SC@DS NPs after 2 and 4Â h incubation, respectively. (c) The measurement of relative uptake on Caco-2 cells after being incubated with the different endocytosis inhibitors. (d) The influences of Pra and Gly-sar on the cellular uptake of DS NPs and SC@DS NPs, respectively. (e) Establishment of Caco-2 cell monolayer models. (f) The CLSM images of different NPs on Caco-2 cell monolayers from apical to basolateral side. (g) TEER values of Caco-2 cell monolayers before and after different incubations. (h) The amount of transepithelial transport of different groups in the basolateral chamber. (i) The TEM images of DS NPs and SC@DS NPs in the basolateral chamber