Fig. 2
Identification and uptake assay of AFs-derived exosomes. A Representative transmission electron microscopic photographs of AFsNPi-Exos and AFsHPi-Exos. The scale bar represents 200 μm. B The diameter distribution of AFsNPi-Exos and AFsHPi-Exos was detected by DLS analysis. C Western blot analysis of exosome-specific markers TSG101, CD9, and CD81 in exosomes secreted by NPi/HPi-induced AFs (AFsNPi-Exos, AFsHPi-Exos). D Representative images of AFsNPi-Exos and AFsHPi-Exos uptake by VSMCs after 12 h incubation. Nuclei stained by DAPI in blue and AFs-Exos stained by PKH26 in red. Scale bar: 50 μm