Fig. 2

ZIF-8 promotes DPSC neural differentiation. (A) Fluorescent images of calcein-labeled DPSCs cells after different treatments of ZIF-8 (0, 2.5, 5, 10, 20 and 50 µg/ml) within B27 medium for 6 days. Trends of DPSCs neural differentiation (mean ± SD): (B) neural-like cell ratio, (C) the neurite number and (D) neurite length. DPSCs were treated with different concentrations of ZIF-8 (0, 2.5, 5, 10, 20 and 50 µg/ml) in B27 nerve differentiation culture for 7 days (E) Immunofluorescence analysis of expression of βIII-tubulin (green) and NeuN (red). Fluorescence intensity quantification of NeuN and βIII-tubulin is given. (F) Western Blot was used to detect the expression of Nestin, βIII-tubulin, GFAP, S100b and β-actin. (G) Quantitative statistics of gray value were given. ***P < 0.001