Fig. 4

ZIF-8 promotes neural differentiation and angiogenesis of DPSCs through MAPK signaling pathways. (A) Heat map showing the top 40 differential gene expression. KEGG analysis of upregulated (B) and downregulated (C) signaling pathways. GO enrichment analysis of upregulated (D) and downregulated (E) signaling pathways. (F) DPSCs were treated with ZIF-8 (0, 2.5, 5, 10, 20 and 50 µg/ml) for 7 days in B27 medium. Western Blot was used to detect the expression of ERK1/2, p-ERK1/2, JNK1, p-JNK1, p38, p-p38 and GAPDH. Quantitative statistics of gray value were given. (G) DPSCs were treated with SP600125 (0 and 40 µM) combined with ZIF-8 (0 and 20 µg/ml). Western Blot was used to detect the expression of p-JNK1, βIII-tubulin, S100b and GAPDH. Quantitative statistics of gray value were given. (H) DPSCs were treated with SB203580 (0 and 20 µM) combined with ZIF-8 (0 and 20 µg/ml). Western Blot was used to detect the expression of p-38, VEGF-a and GAPDH. Quantitative statistics of gray value were given. **P < 0.01; ***P < 0.001