Fig. 5
ZIF-8 promotes neural differentiation and angiogenesis of DPSCs depended on Zn2+ release. (A) Fluorescent images of calcein-labeled DPSCs cells treated with TPEN (0, 0.25, 0.5, 1, 1.5 and 2 µM) with ZIF-8 within (20 µg/ml) in B27 medium for 6 days. Trends of DPSCs neural differentiation (mean ± SD): (B) neural-like cell ratio, (C) the neurite number and (D) neurite length. DPSCs were treated with different concentrations of Zncl2 (0, 0.5, 1, 2, 4, 10 and 20 µM) in B27 nerve differentiation culture for 7 days (E) Immunofluorescence analysis of expression of βIII-tubulin (green) and NeuN (red). Fluorescence intensity quantification of NeuN and βIII-tubulin is given. (F) DPSCs were treated with TPEN (0 and 0.5 µM) combined with ZIF-8 of concentration gradient (0 and 20 µg/ml), the medium was co-culture with HUVECs. Then cells stained with the crystal violet stain for Transwell experiments. The number of transferred cells is quantified. (G) DPSCs cells treated with TPEN(0, 0.1, 0.25 and 0.5 µM) with ZIF-8 within (20 µg/ml) in B27 medium for 7 days. Western Blot was used to detect the expression of p-JNK1, p-38, VEGF-a, βIII-tubulin, and GAPDH. Quantitative statistics of gray value (H) were given. (I) DPSCs were treated with TPEN (0 and 0.5 µM) combined with ZIF-8 or Zncl2 of concentration gradient (0, 0.5, 1 and 2 µM). After 24 h treatment, CCK-8 was used to detect cell viabilities. **P < 0.01; ***P < 0.001