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Table 1 Specificity evaluation of CPA and CPA-Cas 12a sensing strategy

From: CPA-Cas12a-based lateral flow strip for portable assay of Methicillin-resistant Staphylococcus aureus in clinical sample

Species

CPA

CPA-Cas 12a

MRSA

MecA(+) nuc (+)

MecA(+) nuc (+)

S. aureus

MecA(-) nuc (+)

MecA(-) nuc (+)

S. pneumoniae

-

-

E. coli

-

-

P. aeruginosa

-

-

Candidaalbicans

-

-

E. faecalis

-

-

E. cloacae

-

-

Klebsiella oxytoca

-

-

Mixture (S. aureus+)

MecA(-) nuc (+)

MecA(-) nuc (+)

Mixture (S. aureus-)

-

-

The First Chongqing EQC in 2020, No. 4

MecA(+) nuc (+)

MecA(+) nuc (+)

The Second National EQC in 2018, No. 3

MecA(+) nuc (+)

MecA(+) nuc (+)

  1. Note: Mixture (S. aureus+) represents the mixture suspension of the above bacteria containingS. aureus; Mixture (S. aureus-) represents a mixture suspension of the above bacteria that do not containS. aureus. “+” indicates a positive test result. “-” indicates a negative result. EQC, external quality control
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Journal of Nanobiotechnology

ISSN: 1477-3155

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