Fig. 6

Biocompatibility for rBMSCs cultured on mineralized ZIF-8/PCL (mZIF-8/PCL). a) LSCM images showing rBMSCs inoculated on mPCL and mZIF-8/PCL for 3/7-day after Calcein-AM/PI staining. Obviously, mZIF-8/PCL exhibited the superior affinity for cells. b) Difference in cell cytotoxicity of rBMSCs co-cultured onto both groups was not significant in relative to control group. c) rBMSCs morphologies and quantities compared after culture for 3 and 7 days. Red and blue indicate actin filaments and cell nuclei, respectively. d) Cell viability analysis demonstrated the increased rBMSCs proliferation after co-culture using mZIF-8/PCL. e) rBMSCs inoculated on mZIF-8/PCL at 3/24-h post-culture displayed the superior cytoskeletal development, greater cell spreading area and formation of thick stress fibers. f) Cytomorphometry analysis based on images displayed in panel c. g-i) Action expression explored from the images. Actin expression in each cell and cell area was analyzed through densitometry based on confocal microscopic images shown in panel e. *p < 0.05, **p < 0.01, *** p < 0.001 (n = 6)