Fig. 7

In vitro osteogenic properties of rBMSCs cultured with mZIF-8/PCL scaffolds. a) Macroscopic and microscopic observations on mPCL and mZIF-8/PCL after rBMSCs were cultivated within osteogenic induction medium and stained with Von kossa on the 14th day. Right-most panels: mineralization ECM of rBMSCs incubated using scaffold leaching solutions stained by von Kossa on the 14th day. mZIF-8/PCL group displayed a greater number of mineralized nodules from the two experiments. b) Alp and Runx2 gene levels within rBMSCs on the 5th day following culture using mZIF-8/PCL together with Ocn and Opn gene levels within rBMSCs on the 14th day markedly elevated in comparison with mPCL group. c, d) Typical WB images and summary analysis revealed that RUNX2 and ALP expression elevated on the 5th day, while OCN and OPN expression elevated on the 14th day in ZIF-8/PCL group. **p < 0.01, ***p < 0.001 (n = 6)