Fig. 1
Characterizations of network nanocapsules. A Model proteins with different isoelectric points and molecular weights were used in this study. B Size distribution N(dh) and surface ζ-potential distribution I(ζ) of the nanocapsules loaded with model proteins were measured in ultrapure water at a concentration of CNPs = 1 mg/mL. C TEM images of VCNCs. D Number distribution N(dh) and E surface ζ-potential of VCNCs, CNCs, and VNCs were recorded in ultrapure water at a concentration of CNPs = 1 mg/mL. F Time-dependent colloidal stability of VCNCs, CNCs, and VNCs (CNPs = 1 mg/mL) in PBS was measured in terms of hydrodynamic size dh(N). G Relative CAT viability VCAT in VCNCs, CNCs, and free CAT was measured at the same amount of CAT CCAT = 10 μg/mL in PBS for up to 5 d. Data at each time point were normalized to V = 100% at t = 0 d (n = 3). H Time-dependent ratio of anti-BSA absorption of VCNCs, CNCs, and VNCs was measured (n = 3)