Fig. 10
a The tumor growth curves of mice were intravenously treated with PBS, and ILA@Lip (AQ4N: 5 mg kg−1, Lenvatinib: 100 mg kg−1, IR 780: 5 mg kg−1) with or without laser irradiation every 2 days for 3 cycles. Values represent means ± SD, n = 3. ***P < 0.001 vs control. b Body weight changes during the experiment. Values represent means ± SD, n = 3. c Representative bioluminescence images of mice with tumors after treatments. Hepatic and renal function test of d ALT, e AST, f BUN, and g CR. Values represent means ± SD, n = 3. Representative immunofluorescence images of h CD 31 and i HIF-1α staining in 4T1 tumors of mice with different treatments at the experimental endpoint. Scale bar = 50 μm. j TUNEL staining of tumor slices from mice with different treatments. Scale bar = 50 μm. k The immunohistochemical studies of Ki-67. Scale bars = 50 μm. H&E staining of l tumor slices and m major organs from mice after various treatments. Circled areas are the tumor cells. Scale bars = 50 μm (tumor, heart, spleen, lung, kidney); Scale bars = 100 μm (liver)