Fig. 6

In vitro anti-angiogenesis assay. a Representative images of tube-like structures treated by Lenvatinib (2 μg mL⁻¹), AQ4N (2 μg mL⁻¹), free IR 780 (2 μg mL⁻¹) and ILA@Lip (2 μg mL⁻¹) with/without laser after 5 and 10 h. Scale bar = 200 μm. Quantitative analysis of b the average number of nodes and c total branch length after being treated by lenvatinib, AQ4N, IR 780, and ILA@Lip with/without laser after 5 and 10 h. Values represent means ± SD, n = 3. *P < 0.05; **P < 0.01; ***P < 0.001 vs control. d The microscope images show tube formation by HUVEC on Matrixgel after treatment with Lenvatinib (2 μg mL⁻¹), AQ4N (2 μg mL⁻¹), free IR 780 (2 μg mL⁻¹) and ILA@Lip (2 μg mL⁻¹) with/without laser for 5 h. Scale bar = 200 μm. Quantitative analysis of e the average number of nodes and f total branch length formatted after being treated by lenvatinib, AQ4N, IR 780, and ILA@Lip with/without laser after 5 h. Values represent means ± SD, n = 3. ***P < 0.001 vs control