Fig. 1

PCAF expression was significantly elevated in RA patients and CIA mice. (a) The expression of CBP, p300, GCN5 and PCAF in the synovium of OA and RA patients was detected by IHC. Semiquantification of the mean optical density of CBP, p300, GCN5 and PCAF determined by IHC (n = 6). (b) The expression of CBP, p300, GCN5 and PCAF in the synovium of control and CIA mice was detected by IHC. Semiquantification of the mean optical density of CBP, p300, GCN5 and PCAF determined by IHC (n = 5). (c) The expression of PCAF in synovial macrophages of OA and RA patients were detect by IF (CD68 green, PCAF red). Semiquantification of the mean fluorescence intensity of PCAF and CD68 determined by IF (n = 6). (d) The expression of PCAF in synovial macrophages of control and CIA mice were detect by IF (F4/80 green, PCAF red). Semiquantification of the mean fluorescence intensity of PCAF and F4/80 determined by IF (n = 5). (e) The mRNA levels of CBP, p300, GCN5 and PCAF during the polarization of macrophages were determined by qRT‒PCR (n = 3). (f) The protein levels of CBP, p300, GCN5 and PCAF during the M1 polarization of macrophages were determined by western blotting (n = 3). ns indicates P > 0.05, and * indicates P < 0.05