Fig. 2

M1 polarization and arthritis were significantly alleviated via PCAF inhibition in vitro and in vivo. (a) The MFI of HLA-DR was detected in CD68-positive cells via flow cytometric analysis (n = 3). (b) The MFI of HLA-DR was detected in CD68-positive cells via flow cytometric analysis in siRNA assays (n = 3). (c) Schematic of CIA in PCAF-/- mice. (d) Arthritis severity was determined using a visual arthritis scoring system in WT and PCAF-/- mice (n = 6). (e) The general degree of ankle swelling in WT and PCAF-/- mice after CIA induction and corresponding H&E staining of ankle joints excised from the mice are shown. (f) The expression of IL-6, TNF-α and IL-1β in WT and PCAF-/- mice after CIA induction was detected by IHC. Semiquantification of the mean optical density of IL-6, TNF-α and IL-1β determined by IHC (n = 6). (g) The expression of iNOS in synovial macrophages of control and CIA mice were detect by IF (F4/80 green, iNOS red). Semiquantification of the mean fluorescence intensity of iNOS and F4/80 determined by IF (n = 6). ns indicates P > 0.05, and * indicates P < 0.05