Fig. 3

The natural compound garcinol, a PCAF inhibitor, significantly alleviated joint inflammation in CIA mice in vivo. (a) Schematic of CIA mice treated with garcinol. (b) Arthritis severity was evaluated using an arthritis scoring system in CIA mice treated with vector or 10 mg/kg garcinol (n = 6). (c) The degree of ankle swelling in CIA mice treated with vector or 10 mg/kg garcinol and H&E staining of the ankle joints excised from the mice are shown. (d) The expression of IL-6, TNF-α and IL-1β in CIA mice treated with vector or 10 mg/kg garcinol was detected by IHC. Semiquantitation of the mean optical density of IL-6, TNF-α and IL-1β determined by IHC analysis (n = 6). (e) The expression of iNOS in synovial macrophages of CIA mice treated with vector or 10 mg/kg garcinol were detect by IF (F4/80 green, iNOS red). Semiquantification of the mean fluorescence intensity of iNOS and F4/80 determined by IF (n = 6). ns indicates P > 0.05, and * indicates P < 0.05