Fig. 5

PCAF inhibition synergistically suppressed the acetylation of p65 and H3K9. (a) The protein levels of PCAF, p-p65, p65-K310Ac and p65 during the M1 polarization of macrophages were detected by western blotting (n = 3). (b & c) The binding of p65 and PCAF was assessed by co-IP assay (n = 3). (d) The levels of p-p65 and Ac-p65 (K310) were reduced by garcinol treatment (n = 3). (e) The levels of PCAF, p-p65, and Ac-p65 (K310) were reduced by PCAF siRNA knockdown (n = 3). (f) Changes in the abundance of various types of histone acetylation during the M1 polarization of macrophages were detected by western blotting (n = 3). (g) Changes in H3K9Ac, H3K27Ac and H4K16Ac levels after garcinol treatment are shown (n = 3). (h) Changes in H3K9Ac, H3K27Ac and H4K16Ac levels after PCAF siRNA knockdown are shown (n = 3). Changes in the acetylation level of p65 before and after the mutation of different residues (n = 3). (i) Changes in the acetylation level of p65 after PCAF overexpression and p65 mutations. (j) Changes in the binding of p65 and H3K9Ac at the promoters of TNF-α, IL-6 and IL-1β after garcinol treatment (n = 3). ns indicates P > 0.05, and * indicates P < 0.05