Fig. 7

Garcinol-DSNPs ideally targeted inflammatory joints and effectively alleviated arthritis. (a) The time-dependent distribution of DSNPs in images of the whole hind legs of control and CIA mice was evaluated with an IVIS system. (b) Ex vivo distribution of DSNPs in various organs from control and CIA mice. (c) Arthritis severity was evaluated using an arthritis scoring system in CIA mice treated with vector, DSNPS, 10 mg/kg garcinol or 10 mg/kg garcinol-DSNPS (n = 6). (d) The general degree of ankle swelling in CIA mice treated with vector, DSNPs, 10 mg/kg garcinol or 10 mg/kg garcinol-DSNPs and H&E staining of ankle joints excised from the different groups of mice are shown. (e) The expression of IL-6, TNF-α and IL-1β in CIA mice treated with vector, DSNP, 10 mg/kg garcinol or 10 mg/kg garcinol-DSNP was detected by IHC. Semiquantification of the mean optical density of IL-6, TNF-α and IL-1β detected by IHC (n = 6). ns indicates P > 0.05, and * indicates P < 0.05