Table 4 Enzyme responsive nanotechnology designs

TNT-DFO-HA-Gen coating (Yu2020) [44]

HAase-HA enzyme

1. Electrochemical treatment of Ti to form TNT

2. Connect HA to Gen

3. Assembly of HA-Gen and Chi

4. DFO is loaded on TNT

5. Ha-gen-chi seal DFO-Ti surface

HA on TNT surface recognizes HAase enzyme secreted by bacterial to achieve specific touch release

S. aureus

E. coli

1. Inhibit bacterial adhesion

2. Antibacterial drug release

3. Promote osseointegration

TNT-Van-Chic-HAc coating (Yuan2018) [43]

HAase-HA enzyme

1. Electrochemical treatment of Ti to form TNT

2. TNT coated Van

3. Dop modified HA-C and Chi-C

4. LBL assembly of HA-C and Chi-C

5. Dop-hac-Chic film is attached to the surface of TNTS-VAN

HA-c on TNT surface recognizes HAase enzyme secreted by bacterial to achieve specific touch release

S. aureus

1. Inhibit bacterial adhesion

2. Antibacterial drug release

3. Promote osseointegration

PG-PAH LBL@CHI-Ag coating (Ding2019) [46]

V8 enzyme-PG

1. AgNPs were wrapped in CHI

2. PG and PAH were assembled on CHI-Ag surface by LBL

3. LBL@CHI-Ag was deposited on the surface of Ti matrix modified with PDOP

PG on TNT surface recognizes V8 enzyme secreted by bacterial to achieve specific touch release

S. aureus

1. Inhibit bacterial adhesion

2. Antibacterial drug release

3. Promote osteogensis

Ti-GL13K-MMP-9 coating (Fischer2021) [45]

MMP9-CP enzyme-MMP-9

GL13K antimicrobial peptide and MMP9-CP were co-fixed on the surface of Ti

MMP-9 on TNT surface recognizes MMP-CP enzyme secreted by bacterial to achieve specific touch release

S. gordonii

1. GL13K antimicrobial peptides antibacterial

2. Promotes bone formation

Ti-CIP-PLL-Alg coating (Bourgat2021) [47]

Trypsin-PLL

1. CIP combine PLL

2. CIP-PLL mixed with Alg to form nanogel

3. Nanoparticles are coated on TI surface

PLL on TNT surface recognizes trypsin enzyme secreted by bacterial to achieve specific touch release

S. aureus

Antibacterial drug release