Fig. 3

miR-184 is upregulated in aqueous humor-derived exosomes from patients with CSC. A Schematic of whole aqueous humor fluid (AH) and isolated exosome to depict location, distribution, and concentration of miRNAs of interest. B miR-184 detection using probe-based qPCR in aqueous humor fluid of the control and purified exosome from the same aqueous humor sample. Expression of miR-184 isolated from the purified exosomes was significantly higher, indicating that miR-184 is more concentrated in exosomes. C Schematic of tissue culture from a fresh donor eye and media obtained for analysis of the secreted exosomes from the retina and RPE-choroid of the fresh cultured tissues. D miR-184 detection using probe-based qPCR in the retina or RPE-choroid tissue cultured media. miR-184 was significantly higher in RPE-choroid cultured media in the first 36 h after fresh incubation. E Representative multimodal images of a patient with subacute CSC. (i) UWF (ii) SLO-IR (iii) BAF (iv) IRAF (v) OCTA (SCP) (vi) OCTA (DCP) (vii) OCTA (OPL-BRM) (viii) OCTA (choriocapillaris) (ix) OCT at baseline (x) OCT after 1-month treatment of anti-VEGF therapy. F Exosomal miR-184 expression from the aqueous humor of the patient with CSC (E) is measured and relatively quantified compared with the control. Four independently synthesized miRNA cDNAs were amplified with two replicates per cDNA to reduce the technical error. Note the significant elevation of miR-184 expression. The graph values are represented as mean ± standard deviation. Statistical significance indicated as *P < 0.05, **P < 0.01, ***P < 0.001