Fig. 7

Downregulation of STC2 promotes angiogenesis in choroidal endothelial cells. A The STC2 mRNA was quantified in STC2 siRNA transfected (STC2 knock-down) hCECs 24 h after transfection. B-C siSTC2 treated cells were seeded for tube formation at 24 h after transfection. Total loops and branching points were measured for tube formation property parameter at 4 h. Note that significant decrement of total loops and branching points at STC2 knock-down cells. (n = 3 individual assays) D 3D Tube formation assay using STC2 knock-down hCECs at 4 h after seeding. Images were skeletonized, magnified and measured for analysis. E Scratch wound closure assay using siSTC2-treated hCECs. The wound space was measured at 12 h after scratching. F The wound space area was measured (n = 3 individual assays). G-I Individual patient-derived exosomal miR-184 quantification G and STC2 mRNA quantification H of controls and patients with CSC (n = 8 for controls, n = 14 for CSC)