Fig. 9From: Advances in nanomedicines for lymphatic imaging and therapyCopyright © 2014 Elsevier LtdSimplified preparation of nanogel, modified spectral analysis of fluorescent agent, and imaging of mouse axillary lymph nodes before and after front limb injection. Fluorescent (A) and optical (B) images of FDNG (5-AF) selectively entering LVs and SLN. Fluorescent (C) and optical (D) images of FDNG(5-AF) and methylene blue co-injected mice after skin removal. Fluorescent (E) and optical (F) images of dissected SLN and adjacent fat tissues. G Immunohistofluorescence staining of dissected SLN from FDNG(5-AF) treated mouse after 12 h of injection. (H) The partially enlarged image of (G) [117].Back to article page