Fig. 5

IL-1b release and NLRP3 inflammasome activation in macrophages cultured on different types of nanofibers. (A) ELISA was used to determine IL-1b release by BMDMs stimulated with LPS and nigericin. (B) Western blotting analysis of the expression of NLRP3 and related signaling proteins in BMDMs stimulated with LPS and nigericin. (C) Immunofluorescence analysis of ASC oligomerization in BMDMs (ASC: green and nucleus: blue), indicating inflammasome assembly. (D) Proportions of BMDMs with ASC specks. (E) Conceptual graphs of NLRP3 inflammasome activation and function. The data are presented as the mean ± standard deviation of three experimental replicates. Statistical analysis was performed by two-tailed Student’s t test and one-way ANOVA with Tukey’s post hoc analysis. *p < 0.05; **p < 0.01, ***p < 0.001; ns indicates no significance