Fig. 6

Subcutaneous macrophage recruitment, polarization and inflammasome activation. (A) Immunofluorescence analysis of CD86 and CD206, indicating macrophage recruitment and polarization, on different types of subcutaneous membranes (CD86: green, CD206: red, and nucleus: blue). (B) Statistical analysis of the ratios of M2 to M1 macrophages. (C) Statistical analysis of the total number of recruited macrophages. (D) Immunofluorescence images of subcutaneous ASC oligomerization in recruited macrophages (ASC: red, CD68: green, and nucleus: blue). (E) Proportions of macrophages with ASC specks. (F) Conceptual graphs of macrophage polarization. The data are presented as the mean ± standard deviation of three experimental replicates. Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc analysis. *p < 0.05; **p < 0.01, ***p < 0.001; ns indicates no significance