Fig. 2
From: Effective treatment of metastatic sentinel lymph nodes by dual-targeting melittin nanoparticles
In vitro evaluation of cellular uptake and cytotoxicity of MLT-HA-HPPS to 4T1 cells. (a) Flow cytometry (FCM) was performed to analyze and compare uptake of HA-HPPS and MLT-HA-HPPS by 4T1 cells in vitro after exposure to various concentrations for 0.5 h, 1 h, and 3 h. (b) Confocal imaging was performed to compare 4T1 cell uptake of HA-HPPS and MLT-HA-HPPS in vitro after exposure to DiR-BOA (10 μM) for 1 h incubation. (c) Proliferation assays evaluating the cytotoxicity of melittin, HA-HPPS, and MLT-HA-HPPS on 4T1 cells for 3 h. (d) Scatter plot of the FCM assays examining the cytotoxicity of MLT-HA-HPPS in 4T1 cells at different time and concentrations. Data are presented as the mean ± SD. n = 3. **P < 0.01, ***P < 0.001