Fig. 4
From: Effective treatment of metastatic sentinel lymph nodes by dual-targeting melittin nanoparticles
In vivo comparisons of the migratory capabilities of HA-HPPS and MLT-HA-HPPS to popliteal SLN. a Schematic of popliteal SLN (pSLN) tumor metastasis detection after six days of 4T1 cells inoculation. b Representative fluorescence images to observe the migratory capabilities of HA-HPPS and MLT-HA-HPPS to pSLN at 0.5, 3, 6, 12, and 24 h after paracancer injection. c Quantitative analysis of the MFI of DiR-BOA in pSLN. d Six days after hock inoculation of tfRFP-4T1 cells (1 × 106 cells/mouse), fluorescence imaging of resected pSLNs were performed at 6 h after paracancer injection of FITC-labelled HA-HPPS and MLT-HA-HPPS. Red: tfRFP-4T1 cells, green: FITC-labelled nanoparticles. e Quantitative analysis of the MFI of FITC on resected pSLNs. f Confocal microscopy verified the ability of FITC-labelled HA-HPPS and MLT-HA-HPPS to target 4T1 cells in tumor metastasis SLNs. Blue: DAPI, Red: tfRFP-4T1 cells, green: FITC-labelled HA-HPPS and MLT-HA-HPPS. Data are presented as the mean ± SD. n = 3 mice/group. *P < 0.05