Fig. 5
In vivo 2D PA and US images of an orthotopic MB49-Luc tumor before and after administration of GNRs@PEG-Iso4. MB49-Luc cells were implanted orthotopically in the bladder of a mouse. After 14 days, the bladder was imaged by PA and US analysis before and immediately after intravesical instillation of GNRs@PEG-Iso4 (26 nmol Au, ~ 1 × 1011 NPs), and after 15 min of incubation and bladder washing. A A representative axial 2D PA and US images of the entire bladder according to the indicated time of analysis. Grayscale, co-registered US signal; Green, PA signal Bar, 2 mm. B Zoomed images of Panel A and PA spectra in the selected ROIs according to the indicated time of analysis. Asterisk, Tumor; Red and Orange features delineate the ROIs drawn on the apical part of the tumor (ROI 1) and outside the bladder (ROI 2), respectively. Note that the PA spectrum detected in the tumor after installation of GNRs@PEG-Iso4, but not that of the PA signal observed outside the bladder, is very similar to the spectrum obtained with the same nanoparticles dispersed in agar drops (inset). Arrows, λmax: ~ 830 nm