Fig. 3
From: The influence of protein corona on Graphene Oxide: implications for biomedical theranostics
Adapted from Quagliarini et al., Nanomaterials 10.8 (2020): 1482. and Cui et al. Nanoscale Advances 4.18 (2022): 4009–4015
a Confocal microscopy images of MCF-F and MDA-MB 231 cells treated with commercial liposomal doxorubicin (DOX) Doxoves® and DOX-loaded graphene oxide (GO) formulation (GO-DOX). The hystogram plots show the fluorescence intensity of nuclear and cytoplasmic signals in cells related to Doxoves® and GO-DOX complexes. b Phasor fluorescence lifetime imaging microscopy (FLIM) analysis performed on MDA-MB 231 cells treated with free DOX (upper left panel) and GO-DOX (upper right panel). The phasor plots contain cluster of points corresponding to pixels with similar lifetime. The cluster are identified by specific region of interest (ROIs) related to each molecular species (e.g., free DOX with red ROI, DOX attached to biological membranes with green ROI etc.,). In bottom panels, intensity and lifetime images of DOX-treated cells and GO-DOX-treated cells coloured according to the ROIs. c Cell viability of U87, Hela and CasKi cells treated with naked GO and GO incubated with different percentages of human plasma (HP). d Densitometric quantification of HER-2, ERK, and AKT expression, normalized on β-actin, and of pHER-2/HER-2, from three independent experiments; one-way ANOVA test followed by Tukey’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001).