Fig. 2

MSC small extracellular vesicles (sEVs) suppressed LPS-induced inflammation in lung epithelial cells

(A) Fluorescence microscopy analysis of PKH67-labeled MSC sEVs internalization by A549 cells and SAECs. The green labeled MSC sEVs were visible in the perinuclear region of recipient cells. Scale bar = 20 μm

(B-E) mRNA and protein levels of (B) tumor necrosis factor alpha (TNF-α), (C) interleukin-1β (IL-1β), (D) interleukin-6 (IL-6), and chemokines (E) macrophage chemoattractant protein-1 (MCP-1) in A549 cells which were treated with or without MSC sEVs

(F-I) mRNA and protein levels of (F) tumor necrosis factor alpha (TNF-α), (G) interleukin-1β (IL-1β), (H) interleukin-6 (IL-6), and chemokines (I) macrophage chemoattractant protein-1 (MCP-1) in SAECs which were treated with MSC sEVs

Statistical analysis: one-way ANOVA with a Tukey-Kramer post hoc test. * P < 0.05, ** P < 0.01, compared between the Sham group and each treated group (LPS, Sham + sEVs, LPS + sEVs). # P < 0.05, ## P < 0.01, compared between the LPS and the treated group (Sham + sEVs, LPS + sEVs). & P < 0.05, && P < 0.01, compared between the Sham + sEVs and the LPS + sEVs group. n = 3 for mRNA and n = 6 for protein in each group