Fig. 5

miR-223-3p is abundantly expressed in MSC small extracellular vesicles (sEVs) and could target PARP-1

(A) MiRNA expression spectrum in sEVs derived from human umbilical cord Wharton’s jelly-derived MSCs were analyzed by high-throughput sequencing

(B) High expression of miR-223-3p in human umbilical cord Wharton’s jelly-derived MSCs was measured by qRT-PCR

(C) miR-223-3p is also an evolutionarily conserved miRNA.

(D) Bioinformatic analysis predict that the 3′UTRs of PARP-1 are complementary to the miR-223-3p seed region

(E-F) Luciferase activity of PARP-1-wt and PARP-1-mut was determined upon miR-223-3p mimic transfection in (E) A549cells and (F) SAECs (n = 3). Statistical analysis: one-way ANOVA with a Tukey-Kramer post hoc test. ** P < 0.01, compared between the miR-223-3p mimic transfection group and the control group

(G-H) The enrichment of miR-223-3p detected by RNA pull-down assay in (G) A549 cells and (H) SAECs. Statistical analysis: one-way ANOVA with a Tukey-Kramer post hoc test. ** P < 0.01, compared with the Bio-NC group. ## P < 0.01, compared with the Bio-miR-223-3p mut group