Fig. 6

MSC small extracellular vesicles (sEVs) increased miR-223-3p while decreasing the expression of its target gene PARP-1 expression in both lung epithelial cells and lung tissues

(A-C) The levels of miR-223-3p in LPS stimulated (A) A549 cells and (B) SAECs after coculturing with or without MSC sEVs. (C) The levels of miR-223-sp in lung tissues of LPS mice which were treated with or without MSC sEVs. (n = 3)

(D-F) The mRNA levels of PARP-1 in LPS stimulated (D) A549 cells and (E) SAECs after coculturing with or without MSC sEVs. (F) The mRNA levels of PARP-1 in lung tissues of LPS mice which were treated with or without MSC sEVs. (n = 3)

(G-I) The protein levels of PARP-1 in LPS stimulated (G) A549 cells and (H) SAECs after coculturing with or without MSC sEVs. (I) The protein levels of PARP-1 in lung tissues of LPS mice which were treated with or without MSC sEVs. (n = 3). Full-length blots/gels are presented in Supplementary Files 5

Statistical analysis: one-way ANOVA with a Tukey-Kramer post hoc test. ** P < 0.01, compared between the Sham group and each treated group (LPS + Saline, Sham + sEVs, LPS + sEVs). # P < 0.05, ## P < 0.01, compared between the LPS + Saline and the treated group (Sham + sEVs, LPS + sEVs). & P < 0.05, && P < 0.01, compared between the Sham + sEVs and the LPS + sEVs group