Fig. 7

Mesenchymal stem cell small extracellular vesicles (MSC sEVs) treatment suppressed inflammation in lung epithelial cells through the delivery of miR-223-3p

Lung epithelial cells were cocultured with MSC sEVs which were additionally treated with a specific inhibitor targeting miR-223-3p

(A-D) The mRNA and protein expressions of proinflammatory cytokines (A) tumor necrosis factor alpha (TNF-α), (B) interleukin-1β (IL-1β), (C) interleukin-6 (IL-6) and chemokines (D) macrophage chemoattractant protein-1 (MCP-1) in A549 cells were determined by qRT-PCR analysis and enzyme-linked immunosorbent assay (ELISA), respectively. n = 3 for mRNA and n = 6 for protein in each group

(E-H) The mRNA and protein expressions of proinflammatory cytokines (E) tumor necrosis factor alpha (TNF-α), (F) interleukin-1β (IL-1β), (G) interleukin-6 (IL-6) and chemokines (H) macrophage chemoattractant protein-1 (MCP-1) in SAECs were determined by qRT-PCR analysis and enzyme-linked immunosorbent assay (ELISA), respectively. n = 3 for mRNA and n = 6 for protein in each group

(I-J) The mRNA expressions of PARP-1 in (I) A549 cells and (J) SAECs were determined by qRT-PCR analysis. n = 3 per group

(K-N) The protein expressions of PARP-1 in (K, M) A549 cells and (L, N) SAECs were measured by western blot. n = 3 per group. Full-length blots/gels are presented in Supplementary Files 5.

Statistical analysis: one-way ANOVA with a Tukey-Kramer post hoc test. Results are represented as mean ± SD. * P < 0.05, ** P < 0.01, compared with the Sham group. # P < 0.05, ## P < 0.01, compared with the LPS + PBS group. & P < 0.05, && P < 0.01, compared with the LPS + sEVs group. $ P < 0.05, $$ P < 0.01, compared with the LPS + sEVs (223 inhibitor) group