Fig. 8

Mesenchymal stem cell small extracellular vesicles (MSC sEVs) treatment suppressed inflammation in lung tissues of LPS-induced ALI mice through the delivery of miR-223-3p

LPS-induced ALI mice were treated with Saline, MSC sEVs, or miR-223-3p-depleted MSC sEVs.

(A) Sections were stained with hematoxylin and eosin and examined histologically. The representative sections are shown at 200 original magnification, and scale bars are 100 μm. Severity scores of lung injury in the four groups of mice (n = 15). Results are represented as mean ± SD.

(B) Survival rate was monitored for a total of 168 h (7 days) for LPS-induced mice. (*P < 0.05, compared with the LPS + PBS group; #P < 0.05, compared with the LPS + sEVs (223-3p inhibitor)). n = 20 per group

(C-F) The mRNA and protein expressions of proinflammatory cytokines (C) tumor necrosis factor alpha (TNF-α), (D) interleukin-1β (IL-1β), (E) interleukin-6 (IL-6) and chemokines (F) mouse macrophage chemoattractant protein-1 (JE-1) in lung tissues of LPS-induced ALI mice. n = 3 for mRNA and n = 6 for protein in each group

(G-H) (G) The mRNA and (H) protein expressions of PARP-1 in lung tissues of LPS-induced ALI mice. n = 3 per group. Full-length blots/gels are presented in Supplementary Files 5

Statistical analysis: one-way ANOVA with a Tukey-Kramer post hoc test. Results are represented as mean ± SD. * P < 0.05, ** P < 0.01, compared with the Sham group. # P < 0.05, ## P < 0.01, compared with the LPS + PBS group. & P < 0.05, && P < 0.01, compared with the LPS + sEVs group. $ P < 0.05, $$ P < 0.01, compared with the LPS + sEVs (223-3p inhibitor) group