Fig. 9

Inhibition of PARP-1 induce mesenchymal stem cell small extracellular vesicles (MSC sEVs) like positive effects on lung epithelial cells

Lung epithelial cells were cocultured with MSC sEVs or siPARP-1.

(A-B) The inhibitory efficiency of the siRNAs targeting PARP-1 in (A) A549 cells and (B) SAECs was verified by qRT-PCR. n = 3 per group

(C-F) The mRNA and protein expressions of proinflammatory cytokines (C) tumor necrosis factor alpha (TNF-α), (D) interleukin-1β (IL-1β), (E) interleukin-6 (IL-6) and chemokines (F) macrophage chemoattractant protein-1 (MCP-1) in A549 cells

(G-J) The mRNA and protein expressions of proinflammatory cytokines (G) tumor necrosis factor alpha (TNF-α), (H) interleukin-1β (IL-1β), (I) interleukin-6 (IL-6) and chemokines (J) macrophage chemoattractant protein-1 (MCP-1) in SAECs. n = 3 for mRNA and n = 6 for protein in each group

Statistical analysis: one-way ANOVA with a Tukey-Kramer post hoc test

For (A) and (B), ** P < 0.01, compared with the con siRNA group. For (C-J), * P < 0.05, ** P < 0.01, compared with the control group. # P < 0.05, ## P < 0.01, compared with the LPS + PBS group. & P < 0.05, && P < 0.01, compared with the LPS + sEVs group. $ P < 0.05, $$ P < 0.01, compared with the LPS + siPRAP-1 group. Results are represented as mean ± SD.