Fig. 2

A The preparation diagram of MM@MTX NPs. B TEM and the DLS measured the size of MTX NPs (Bar = 200 nm). C FT-IR and D XRD spectrum of free MTX, blank NPs and MTX NPs. E TEM and the DLS measured the size of MM@MTX NPs (Bar = 200 nm). F Zeta potential of MM, MTX NPs and MM@MTX NPs. G Western blot results of CCR2 and CD47 in macrophages, MM and MM@MTX NPs. H Size and I PDI of MM@MTX NPs for 6 d in PBS (pH 7.4) (n = 3). J In vitro release of MTX from MTX NPs and MM@MTX NPs at PBS pH 7.4 with cholesterol or not, free MTX as control (n = 3)