Fig. 3

The figures for study design, HME and BME up-taken by C2C12cells, and effects of HME and BME on C2C12 cells (myotubes) differentiation. (A): The figures for HME/BME co-cultured with C2C12 cells, 3,3’-dioctadecyloxacarbocyanine perchlorate (DiO)-stained myotubes emit green fluorescence, Hoechst-stained nuclei emit blue fluorescence, and 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI)-stained EVs emit orange-red fluorescence. (B): Experimental design for cell culture. (C): Representative Jenner-Giemsa staining images of myotubes. (D-E): Effects of HME and BME on the length and diameter of C2C12 myotubes. (F-G): Myotubes densities are calculated as the sum of pixels attributed to tones 0–60. Data are presented as mean ± SE, the * indicates p < 0.05