Fig. 4

Effects of HME and BME on AKT-mTOR-P70S6K-4EBP1 pathway, AMPK-SIRT1-PGC-1α pathway and regulation of myogenic regulatory factors in C2C12 cells. (A): Figures for Western blotting detection, where C1-C6 lanes for control group samples, H1-H6 lanes for samples intervened by HME, and B1-B6 lanes for samples intervened by BME. (B-E): Protein levels of p-Akt/Akt, p-mTOR/mTOR, p-P70S6K/P70S6K, and p-4E-BP1/4E-BP1 in the C2C12 cells. (F-H): Protein levels of p-AMPK/AMPK, SIRT1, and PGC-1α. (I-K): Protein levels of myogenic regulatory factors Myog, Myf5, and Myod1 in the C2C12 cells. Data are presented as mean ± SE, the * indicates p < 0.05