Fig. 3
In vitro inflammatory factors and ROS of C6 NE in RAW264.7 cells. The expression of TNF-α (A), IL-6 (B), IL-10 (C) was determined by qRT-PCR after treatment of RAW264.7 cells with different preparations stimulated by lipopolysaccharide. Data are shown as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs LPS group. D CLSM images of RAW264.7 cells with different treatments. Green: ROS. Scale bar were 50 μm. E Fluorescence intensity of DCFDA in RAW264.7 cells with different treatments. ***p < 0.001