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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Functional molecule-mediated assembled copper nanozymes for diabetic wound healing

Fig. 3

In vitro biocompatibility and antibacterial activity of Cu-DCA NZs. (A) The viability of HUVECs after different treatments (1.0 W cm− 2, 5 min). (B) Calcein/PI cell viability fluorescence images of HUVECs with different treatments (1.0 W cm− 2, 5 min). (C) Cells uptake of Cu-DCA NZs with different times. (D) RDPP probe detecting intracellular oxygen generation level of HUVECs after different treatments. (E) DCFH-DA assay detecting intracellular ROS level of HUVECs after different treatments. (F) Fluorescence intensity of intracellular oxygen generation level. (G) Fluorescence intensity of intracellular ROS level. (I: PBS, II: H2O2, III: DCA + H2O2, IV: Cu-DCA NZs + H2O2, V: Cu-DCA NZs + H2O2 + NIR). (H) The viability of bacterial with different treatments (1.0 W cm− 2, 5 min). Data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001

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