Fig. 4

W-Exo-L@GelMA suppresses the IL-1β-induced transcriptomic responses related to catabolic effect, inflammation, and immune response. A Principal component analysis of the overall transcriptome of each sample. B Scatter plots of the differentially expressed genes (DEGs) in the comparison between the IL-1β and VEH groups or between the W-Exo-L@GelMA/IL-1β and IL-1β groups. The up- or down-regulated genes are indicated in red or blue color. The top 20 DEGs in the ranked gene list sorted in descending order of expression variance values are labeled by official gene symbols and the rescued genes are labeled in bold. Expression variance is defined by the absolute value of the difference in normalized gene expression (FPKM). C Overlap of the DEGs in each comparison. D The scatter plot of the completely-rescued (Type-1) and partially-rescued (Type-2) genes. E Top 30 IL-1β-induced or suppressed genes notably rescued by W-Exo-L@GelMA. F KEGG pathway analysis of the rescued genes (up-regulated by IL-1β and down-regulated by W-Exo-L@GelMA). G qPCR validations of the key rescued genes. Data represent mean ± SD; N = 3/group. **P < 0.01 by Student’s t-test