Fig. 6

Low dose wound healing activity and histological evaluation of wound sections in vivo. (A) Representative photographs of the rat skin wound healing treated with HSA NPs and HSA-bFGF NPs, compared to NT at day 0, 3, 6, 9, 12, and 14. Dosage of loaded bFGF in HSA-bFGF NP was 3 µg. The boundary between hairy area and the non-hairy area of skin was marked (dashed line, red). Scale bars, 5 mm. (B) Quantitatively analyzed wound size reduction profile. n = 3 to 5. (C) Representative H&E staining images of the wound skin upon different treatments. Panniculus gap was presented as dashed lines and arrows (black). Thickness of regenerated skin treated with HSA-bFGF NPs was thicker compared to other groups. Scale bars, 1 mm. (D) Magnified H&E staining images of each skin designated as a solid box (blue) in (C). Though hyperproliferative epidermis layer shows not completely recover the wound, the wound was shown to be in the process of recovering. Scale bars, 100 μm