Fig. 3

Nanoinjection of IgG­AF647 into GPE-86 cells via the ENI platform. a Loading of the AF647-tagged antibodies into the Au-coated NTs. Scale bar: 20 μm. b Comparison of the confocal microscopy images before (i) and after (ii) an intentional induced photobleaching. Scale bars: 10 μm. c Confocal microscopy images demonstrating the insertion and accumulation of IgG­AF647 into GPE-86 cells after the nanoscale-EP using two voltage amplitudes (5, 10 V) and the comparison to the non-electroporated cells (0 V). Scale bars: 10 μm. d Quantification of the percentage of cells containing IgG­AF647. Error bars indicate ± SDs, n = 3, **P ≤ 0.005, ***P = 0.0001, one-way ANOVA.