Fig. 4

Nanoinjection of Cy5-mRNA-GFP into GPE-86 cells. a Flow cytometry analysis of GPE-86 cells (Negative control (Neg.), 0 V, and 10 V) detached from NTs pre-loaded with Cy5-mRNA-GFP with circle gating indicating Cy5⁺GFP⁺ population. b Quantification of the percentage of cells exhibiting both Cy5 and GFP signals. Error bars indicate ± SDs, n = 3, ***P = 0.0003, one-way ANOVA. c Confocal microscopy images demonstrating the insertion of Cy5-mRNAs into GPE-86 cells and the subsequent GFP expression for 0 and 10 V after 6 h. Scale bars: 10 μm