Fig. 2
Reprint with permission from [61]
RBCs as APCs promote immune memory and epitope spreading, and harness endogenous T cells. a mice with EG7.OVA tumors were first treated with naïve OT-1 cells, and dosed with mRBC-OVA-4-1BBL-IL-12. Then the survivors were rechallenged on day 66 with EG7.OVA. The control group chose age-matched naïve mice treated on day 65 with OT-1 cells 1 day before challenge with EG7.OVA cells. b All previously cured mice rejected EG7.OVA rechallenge. c OT-1 and endogenous OVA-specific T cells both expressed in peripheral blood 10 days after EG7.OVA rechallenge. d, e OT-1 and endogenous OVA-specific T-cell numbers in peripheral blood were significantly high expression 10 days after rechallenge. f–h mRBC-OVA-4-1BBL-IL12 promotes epitope spreading.