Fig. 2

Characterization of HACB/BCNU NPs. A The hydrodynamic particle size of HACB NPs measured by dynamic light scattering. B TEM image of HACB NPs. Scale bars are 500 nm (left) and 200 nm (right). C The stability of HACB NPs. D The particle size and PDI of HACB/BCNU NPs. E Zeta potential of HACB/BCNU NPs. F The hydrodynamic particle size of HACB/BCNU NPs. G TEM image of HACB/BCNU NPs. Scale bars are 500 nm (left) and 200 nm (right). H The azo-benzene bond absorption changes of HACB NPs under reduction (Na₂S₂O₄) at different time points. I The hydrodynamic particle size of HACB NPs after Na₂S₂O₄ incubation. J TEM images of HACB NPs after Na₂S₂O₄ or esterase incubation. Scale bars are 500 nm (left) and 200 nm (right). K The hydrodynamic particle size of HACB NPs after esterase incubation. L In vitro Cou6 release behavior of HACB/Cou6 NPs. Data are shown as mean ± S. D. (n = 3)