Fig. 5
Cytotoxicity assay in vitro. A, B Survival rate of HeLa cells (A) and b End. 3 cells (B) treated with HACB NPs. C Colony formation images and colony formation rate were performed with HeLa cells after treatment. D–F The fluorescent images and dead population of HeLa cells after treatment observed by fluorescence microscope (×100). E Drug concentration of BCNU in each group is 0.1 mM; F Drug concentration of BCNU in each group is 0.3 mM. I Control, II BCNU, III BCNU + O6-BG, IV HAB/BCNU NPs, V HACB/BCNU NPs. Data are shown as mean ± S. D. (n = 3), *p < 0.05, **p < 0.01 and ***p < 0.001